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During a biopsy, a small sample of tissue is taken from the suspicious area. This tissue is then sent to a pathologist who examines it under a microscope. This examination is critical for determining whether cancer cells are present and, if so, what kind of cancer it is. The pathologist looks at the cells' characteristics, including their size, shape, and how they are arranged. These details help to distinguish between different types of cancer. If cancer is found, the tissue sample undergoes further testing to determine its HER2 status, as well as hormone receptor status (ER and PR). This testing is a game-changer. It helps to clarify the cancer’s nature and guide treatment decisions. Techniques like immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) are used to assess the HER2 protein levels. These tests provide the essential information about whether the cancer is **HER2-negative** or HER2-positive.
