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Another important consideration is the potential for silencing of the CMV promoter over time. Although CMV promoters are generally considered to be strong and constitutive, they can be subject to epigenetic modifications that reduce their activity. This can lead to a decrease in the expression of the reprogramming factors and a loss of pluripotency. To address this issue, researchers may use epigenetic modifiers or alternative promoter sequences that are less prone to silencing.
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